Effect of Ascorbic Acid in Vivo on Labeling of Red Cells by Radioactive Sodium Chromate.
نویسندگان
چکیده
S INCE THE INITIAL work of Gray and Sterling,”2 the determination of red cell mass with radioactive sodium chromate has undergone several modifications. Read’ developed the technic of adding ascorbic acid in vitro to the blood-sodium chromate mixture to reduce chromate to trivalent chromium and prevent further uptake by red cells. The inhibition of chromium uptake by ascorbic acid supported the concept of Cray’ that a reducing agent prevents complete tagging of erythrocytes by chromate in vivo. In 1956, Cooper et al.,4 suggested that radioactive sodium chromate could be mixed directly with blood in acid citrate dextrose (ACD ) , and that this mixture led to a higher degree of red cell labeling than when either heparin or sodium citrate alone was used. Since ACD solution did not materially reduce the chromate ion within the first few hours of mixing, these authors added sodium chromate to the ACD solution prior to addition of whole blood. In 1957, Cunningham et al.5 stated that ACD solution does reduce chromate and that such mixtures could not be used effectively longer than 4 hours after mixing. By tracer technics and spectrophotometric analysis of mixtures of ACD and K2Cr51O4, these authors documented reduction of chromate by acid citrate dextrose formation to a chromic-citrate complex. In the course of doing clinical blood volumes we found on several occasions that the per cent of red cells labeled in vitro was decreased markedly from the approximate 90 per cent expected. Further investigation indicated that in vivo concentrations of ascorbic acid affected the labeling of human red cells in vitro.
منابع مشابه
Radioactive sodium chromate for the study of survival of red blood cells. I. The effect of radioactive sodium chromate on red cells.
متن کامل
P-8: The Protective Effect of Ascorbic Acid on The Germ Cells Population and Daily Sperm Production in Mice Treated with Sodium Arsenite
Background Oxidative stress has been suggested to be a major cause of reproductive failure. Arsenic as an environmental toxicant can exert malformation in the reproductive system by inducing oxidative stress. Ascorbic acid, a potent antioxidant, is able to restrict oxidative stress. The aim of this study was to investigate the adverse effect of sodium arsenite on the germ cells population and d...
متن کاملEffect of ascorbic acid on DNA damage, cytotoxicity, glutathione reductase, and formation of paramagnetic chromium in Chinese hamster V-79 cells treated with sodium chromate(VI).
The effect of pretreatment with ascorbic acid (vitamin C) on chromate-induced DNA damage, cytotoxicity, and enzyme inhibition as well as on the cellular reduction of chromium(VI) was investigated using Chinese hamster V-79 cells. Cellular pretreatment with nontoxic levels of 1 mM ascorbic acid for 24 h prior to exposure resulted in a significant increase (1.7-fold) in cellular levels of this vi...
متن کاملThe Inhibitory Effects of Ascorbic Acid, ?-Tocopherol, and Sodium Selenite on Proliferation of Breast Cancer Cell Lines
The role of antioxidants in prevention and treatment of cancers have been reported by several studies. In our investigation we studied the effects of ascorbic acid, ?-tocopherol, and sodium selenite on proliferation of two breast cancer cell lines: T47D (estrogen-receptor positive) and MDA-MB-231 (estrogen-receptor negative). We also used 17-?-estradiol as positive control for proliferation of ...
متن کاملThe Inhibitory Effects of Ascorbic Acid, ?-Tocopherol, and Sodium Selenite on Proliferation of Breast Cancer Cell Lines
The role of antioxidants in prevention and treatment of cancers have been reported by several studies. In our investigation we studied the effects of ascorbic acid, ?-tocopherol, and sodium selenite on proliferation of two breast cancer cell lines: T47D (estrogen-receptor positive) and MDA-MB-231 (estrogen-receptor negative). We also used 17-?-estradiol as positive control for proliferation of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Blood
دوره 22 شماره
صفحات -
تاریخ انتشار 1963